orf2 1 gene Search Results


orf2 1 gene  (New England Biolabs)
99
New England Biolabs orf2 1 gene
Alignment of the nucleotide sequences of the codon-optimized truncated <t>ORF2</t> gene and the original truncated ORF2 gene by CLC sequence viewer 6.5.3 (CLC bio, Aarhus, Denmark). Abbreviaition: ORF, open reading frame.
Orf2 1 Gene, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/orf2+1+gene/NdeI/pmc03948824-51-9-22
Average 99 stars, based on 1 article reviews
orf2 1 gene - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
c11orf21 crispr/cas9 ko plasmid  (Santa Cruz Biotechnology)
N/A
Target species: human. CRISPR/Cas9 KO Plasmids consists of C11orf21-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double
   Buy from Supplier
c1orf216 double nickase plasmid  (Santa Cruz Biotechnology)
N/A
Target species: human. CRISPR/Cas9 KO Plasmids consists of C1orf216-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double
   Buy from Supplier
c1orf216 crispr activation plasmid  (Santa Cruz Biotechnology)
N/A
Target species: human. CRISPR/Cas9 KO Plasmids consists of C1orf216-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double
   Buy from Supplier
c1orf212 shrna (h) lentiviral particles  (Santa Cruz Biotechnology)
N/A
Target species: human. Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of C1orf212 gene silencing results, individual duplex components or plasmids are also available
   Buy from Supplier
c4orf21 shrna (h) lentiviral particles  (Santa Cruz Biotechnology)
N/A
Target species: human. Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of C4orf21 gene silencing results, individual duplex components or plasmids are also available
   Buy from Supplier
c6orf218 shrna (h) lentiviral particles  (Santa Cruz Biotechnology)
N/A
Target species: human. Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of C6orf218 gene silencing results, individual duplex components or plasmids are also available
   Buy from Supplier
c1orf216 recombinant protein antigen  (Novus Biologicals)
N/A
C1orf216 Recombinant Protein Antigen
   Buy from Supplier
cxorf21 recombinant protein antigen  (Novus Biologicals)
N/A
The CXorf21 Recombinant Protein Antigen from Novus Biologicals is derived from E coli The CXorf21 Recombinant Protein Antigen has been validated for the following applications Antibody Competition
   Buy from Supplier
c1orf215 human qpcr primer pair  (OriGene)
N/A
qSTAR qPCR primer pairs against Homo sapiens gene C1orf215
   Buy from Supplier
c1orf212 lentiviral activation particles  (Santa Cruz Biotechnology)
N/A
Target species: human. CRISPR/Cas9 KO Plasmids consists of C1orf212-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double
   Buy from Supplier
c1orf216 hdr plasmid  (Santa Cruz Biotechnology)
N/A
Target species: human. CRISPR/Cas9 KO Plasmids consists of C1orf216-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double
   Buy from Supplier

Image Search Results


Alignment of the nucleotide sequences of the codon-optimized truncated ORF2 gene and the original truncated ORF2 gene by CLC sequence viewer 6.5.3 (CLC bio, Aarhus, Denmark). Abbreviaition: ORF, open reading frame.

Journal: Annals of Laboratory Medicine

Article Title: Development of Enzyme-Linked Immunosorbent Assays Using 2 Truncated ORF2 Proteins for Detection of IgG Antibodies Against Hepatitis E Virus

doi: 10.3343/alm.2014.34.2.118

Figure Lengend Snippet: Alignment of the nucleotide sequences of the codon-optimized truncated ORF2 gene and the original truncated ORF2 gene by CLC sequence viewer 6.5.3 (CLC bio, Aarhus, Denmark). Abbreviaition: ORF, open reading frame.

Article Snippet: The pBluescript II SK (+) vector carrying the optimized ORF2.1 gene (pBluescript II SK-ORF2.1) was digested by NdeI and XhoI restriction enzymes (New England BioLab, Ipswich, MA, USA).

Techniques: Sequencing

PCR amplification of ORF2.1 (amino acids 112-660) and ORF2.2 (amino acids 112-608) with T7 promoter and T7 terminator primers. Lanes 1 and 7, 1-kb DNA ladder (Vivantis, Selangor Darul Ehsan, Malaysia); Lanes 2 and 3, amplified ORF2.1 gene; Lanes 4-6, amplified ORF2.2 gene. Abbreviation: ORF, open reading frame.

Journal: Annals of Laboratory Medicine

Article Title: Development of Enzyme-Linked Immunosorbent Assays Using 2 Truncated ORF2 Proteins for Detection of IgG Antibodies Against Hepatitis E Virus

doi: 10.3343/alm.2014.34.2.118

Figure Lengend Snippet: PCR amplification of ORF2.1 (amino acids 112-660) and ORF2.2 (amino acids 112-608) with T7 promoter and T7 terminator primers. Lanes 1 and 7, 1-kb DNA ladder (Vivantis, Selangor Darul Ehsan, Malaysia); Lanes 2 and 3, amplified ORF2.1 gene; Lanes 4-6, amplified ORF2.2 gene. Abbreviation: ORF, open reading frame.

Article Snippet: The pBluescript II SK (+) vector carrying the optimized ORF2.1 gene (pBluescript II SK-ORF2.1) was digested by NdeI and XhoI restriction enzymes (New England BioLab, Ipswich, MA, USA).

Techniques: Amplification

Proteins expressed using pET30a-ORF2.1 recombinant expression vector and pET30a-ORF2.2 recombinant expression vector in E. coli BL21 (DE3) competent cells. The expressed and purified proteins were analyzed by 10% SDS-PAGE and stained with Coomassie Brilliant Blue R250. The ORF2.1 and ORF2.2 proteins bands are visualized at approximately 60 and 55 kDa, respectively. Lanes 1-4, purified eluates of ORF2.2 protein from the Ni column; Lane 5, washing step; Lane 6, flow-through; Lane M, pre-stained protein ladder (Fermentas, Vilnius, Lithuania); Lane 7-12, purified eluates of ORF2.1 protein from the Ni column; Lane 13, washing step; Lane 14, flow-through. Abbreviations: ORF, open reading frame; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Journal: Annals of Laboratory Medicine

Article Title: Development of Enzyme-Linked Immunosorbent Assays Using 2 Truncated ORF2 Proteins for Detection of IgG Antibodies Against Hepatitis E Virus

doi: 10.3343/alm.2014.34.2.118

Figure Lengend Snippet: Proteins expressed using pET30a-ORF2.1 recombinant expression vector and pET30a-ORF2.2 recombinant expression vector in E. coli BL21 (DE3) competent cells. The expressed and purified proteins were analyzed by 10% SDS-PAGE and stained with Coomassie Brilliant Blue R250. The ORF2.1 and ORF2.2 proteins bands are visualized at approximately 60 and 55 kDa, respectively. Lanes 1-4, purified eluates of ORF2.2 protein from the Ni column; Lane 5, washing step; Lane 6, flow-through; Lane M, pre-stained protein ladder (Fermentas, Vilnius, Lithuania); Lane 7-12, purified eluates of ORF2.1 protein from the Ni column; Lane 13, washing step; Lane 14, flow-through. Abbreviations: ORF, open reading frame; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Article Snippet: The pBluescript II SK (+) vector carrying the optimized ORF2.1 gene (pBluescript II SK-ORF2.1) was digested by NdeI and XhoI restriction enzymes (New England BioLab, Ipswich, MA, USA).

Techniques: Recombinant, Expressing, Plasmid Preparation, Purification, SDS Page, Staining, Polyacrylamide Gel Electrophoresis

Western blot analysis of the expressed and purified ORF2.1 and ORF2.2 protein in E. coli . (A) The ORF2.1 protein band is visualized at approximately 60 kDa. Lane 1, induction for 2 hr; Lane 2, induction for 4 hr; Lane 3, uninduced control; Lane 4, BL21 control; Lane M, pre-stained protein ladder (Cinagen, Tehran, Iran). (B) The ORF2 protein band is visualized at approximately 55 kDa. Lane M, pre-stained protein ladder (Cinagen, Tehran, Iran); Lane 1, uninduced control; Lane 2, induction for 4 hr; Lane 3, flow-through; Lane 4, wash; Lane 5-7, the first, the second, and the third eluates. Abbreviation: ORF, open reading frame.

Journal: Annals of Laboratory Medicine

Article Title: Development of Enzyme-Linked Immunosorbent Assays Using 2 Truncated ORF2 Proteins for Detection of IgG Antibodies Against Hepatitis E Virus

doi: 10.3343/alm.2014.34.2.118

Figure Lengend Snippet: Western blot analysis of the expressed and purified ORF2.1 and ORF2.2 protein in E. coli . (A) The ORF2.1 protein band is visualized at approximately 60 kDa. Lane 1, induction for 2 hr; Lane 2, induction for 4 hr; Lane 3, uninduced control; Lane 4, BL21 control; Lane M, pre-stained protein ladder (Cinagen, Tehran, Iran). (B) The ORF2 protein band is visualized at approximately 55 kDa. Lane M, pre-stained protein ladder (Cinagen, Tehran, Iran); Lane 1, uninduced control; Lane 2, induction for 4 hr; Lane 3, flow-through; Lane 4, wash; Lane 5-7, the first, the second, and the third eluates. Abbreviation: ORF, open reading frame.

Article Snippet: The pBluescript II SK (+) vector carrying the optimized ORF2.1 gene (pBluescript II SK-ORF2.1) was digested by NdeI and XhoI restriction enzymes (New England BioLab, Ipswich, MA, USA).

Techniques: Western Blot, Purification, Staining

Comparison of the immunoreactivities of the 2 in-house ELISAs and DIA.PRO HEV IgG ELISA kit. Data are expressed as OD values of the positive and negative serum samples. The cut-off value is indicated for each assay by a horizontal line (0.35 for the in-house ELISA based on ORF2.1, 0.4 for the in-house ELISA based on ORF2.2, and 0.37 for DIA.PRO HEV IgG ELISA kit). Abbreviations: ORF, open reading frame; OD, optical density.

Journal: Annals of Laboratory Medicine

Article Title: Development of Enzyme-Linked Immunosorbent Assays Using 2 Truncated ORF2 Proteins for Detection of IgG Antibodies Against Hepatitis E Virus

doi: 10.3343/alm.2014.34.2.118

Figure Lengend Snippet: Comparison of the immunoreactivities of the 2 in-house ELISAs and DIA.PRO HEV IgG ELISA kit. Data are expressed as OD values of the positive and negative serum samples. The cut-off value is indicated for each assay by a horizontal line (0.35 for the in-house ELISA based on ORF2.1, 0.4 for the in-house ELISA based on ORF2.2, and 0.37 for DIA.PRO HEV IgG ELISA kit). Abbreviations: ORF, open reading frame; OD, optical density.

Article Snippet: The pBluescript II SK (+) vector carrying the optimized ORF2.1 gene (pBluescript II SK-ORF2.1) was digested by NdeI and XhoI restriction enzymes (New England BioLab, Ipswich, MA, USA).

Techniques: Enzyme-linked Immunosorbent Assay